Flavonoids were isolated from flowers of Salvia leucantha using chromatography separation techniques. The structure of the isolated compound was analyzed by chemical tests and spectroscopic methods such as UV, 1H-NMR, and 13C-NMR. The spectral data’s proved its structure confirmed as Quercetin-3-O- α-L- rhamnopyranoside -2”- gallate. The present study was to evaluate the agar diffusion antibacterial against gram positive and gram negative microorganisms and wound healing activities to excision wound model in albino rats, respectively. The highest inhibitory zone was observed in S. aurous (28mm), and E. coli (24mm). The results revealed most valuable information and also support the continued sustainable use of Quercetin-3-O- α-L- rhamnopyranoside -2”- gallate isolated from Salvia leucantha in traditional system of medicine for future development of new antibiotic agents.