In practical video storage and distribution systems, video sequences are stored and transmitted in a compressed format. During compression, the video frame is transformed from the spatial domain into frequency domain. Therefore, video content authentication methods need to be robust to lossy compression. This paper proposes a novel semi-fragile watermarking video content authentication based on a hybrid feature, consisting of gray threshold and relative total variation edge feature. The watermark is encrypted and embedded into the principal diagonal of 8×8 DCT coefficients. At the receiver side, when a part of the watermarked video is tampered, the proposed approach can locate these tampered areas. The experiment demonstrates the proposed method can distinguish malicious attacks from the legitimate common alterations, such as H.264 lossy compression, Gaussian noise and Gaussian filtering. It has outstanding capability to robust to H.264 compression and to detect malicious attacks. The experiment shows that the proposed method has higher recall and precision than the current approaches.