嶺南野菊 , 學名為 Vernoniapatula (Dryand. ) Merr. , 菊科(compositae) , 斑鳩菊屬(Vemonia) , 別名 : 雙柳廣、狗仔花，分布地: 熱帶地區。大多分布於台灣南部。文獻指出嶺南野菊的乙醇萃取物，在抗發炎 (anti-inflammatory)及抗氧化(antioxidant)活性實驗中，具有劑量依賴性效果，但目前在抗癌活性相關文獻較 少。本研究將分為二部分，來探討嶺南野菊抗癌活性部分，在第一部分為將中草藥嶺南野菊乾草以水蒸顧 萃取，得到嶺南野菊精油萃取物 ;在第二部分由細胞實驗應用於細胞存活率分析、流式細胞儀分析細胞週 期 , 以 MTT 分析(3-(4,5-크^部714비3201ᅵ2-71)ᅵ2,5-邮^ bromide)作細胞存活率分析方法 。 實驗中細胞包括骨肉瘤細胞株 143B 和 MG-63，嶺南精油萃取物作用濃度分別 : 0' 12.5、25、50、100、200 pg/mL ； 實驗結果顯示 : 加A萃取物 24 小時後，其半致死劑量各為 103.87±7.80ig/inL)、57.32±5.0(Hg/mL)。利用流 式細胞儀(Flow cytometry)觀察 143B 細胞 6、12 及 24 小時細胞週期 ; 結果發現 : 精油萃取物處理組的細胞 週期，作用時間增加時 G2/M arrest 有明顯的增加趨勢 。 顯示嶺南野菊精油萃取物確實含有抑制癌細胞生長 的成分，未來將針對嶺南野菊精油粗萃物做進一步的純化分離找出有效的抑制癌細胞生長分子。

Vernonia patula is one of the plants of Compᄋsitae family, it is used to clear away dampness-heat, remove blood stasis, relieve swelling and detoxify the poisons. In folk medicine, it is used to treat common cold, acute gastroenteritis, malaria, mastitis, liver disease and headache. It is also externally applied to treat herpes, eczema, urticarial etc. The objectives of this study were to investigate the effects of various concentrations of Vernonia patula oil in osteosarcoma cells viability, therefore, 143B and MG-63 was investigated by MTT assay. The experimental concentrations of Vernonia patula oil used in the study were 0，12.5, 25，50，100，200 (xg/mL. When the cells were treated with high concentrations of Vernonia patula oil (200 fig/ml) for 24 hours the inhibition percentages were significantly higher than that of the control(p < 0.05). Our results demonstrated the Vernonia patula oil could inhibit the growth of 143B and MG-63 cells. Results showed that half maximal inhibitory concentration value (IC50) were 103.87士7.8 (143B )、57.32 5.0 (MG-63) (^g/mL), and also induced apoptosis through by cell DNA condensation and G2/M arrest in 143B cell.