台灣為海島型國家，海鮮類食品於日常飲食中相當普遍，其中也包括甲殼類。為了解甲殼類中不同部位 之重金屬鎘 (Cd)、鉛 (Pb)、砷 (As) 及汞 (Hg) 累積特徵，利用微波輔助酸消化法 (Microwave assisted acid digestion) 進行樣品前處理，配合感應耦合電漿質譜法 (Inductively coupled plasma mass spectrometry, ICP-MS) 檢測，鎘、 鉛、砷及汞之方法定量極限分別為0.02、0.02、0.05及0.05 μg/g。取牡蠣粉、角鯊魚肝粉、魚肉粉等標準參考物 質進行方法驗證，五重複結果皆能符合標示值。將此方法應用於市售螃蟹九隻、龍蝦四隻、蝦24隻，分析其 各部位重金屬含量，因檢體來源與品種差異，除了汞含量在檢體各部位中皆低於定量極限外，砷、鉛及鎘含 量在個體間差異甚大。螃蟹中鉛有68.0 ± 27.4%分布於鰓(含量0.14~0.54 μg/g)；鎘有90.10 ± 5.40%分布於蟹膏 (含 量0.02~10.62 μg/g)，顯著高於其他部位。龍蝦中鉛僅在龍蝦膏檢出 (含量N.D.~0.07 μg/g)；鎘有94.30 ± 7.64%分 布於龍蝦膏 (含量8.9~21.6 μg/g)，顯著高於其他部位。蝦中鉛僅在頭胸部 (包含內臟與頭部肌肉) 檢出，含量 N.D.~0.07 μg/g；鎘僅在頭胸部檢出，含量N.D.~0.76 μg/g。研究結果顯示龍蝦膏及蟹膏鎘含量偏高，建議國人 儘量減少攝食此高風險部位。

Since Taiwan is an island country, seafood is very common in daily diet, including crustaceans. In order to obtain the accumulation characteristics of cadmium (Cd), lead (Pb), arsenic (As), and mercury (Hg) in different tissues of crustaceans, microwave assisted acid digestion was used for sample pretreatment and heavy-metal concentrations were detected by inductively coupled plasma mass spectrometry (ICPMS). Limits of quantification (LOQs) of Cd, Pb, As, and Hg were 0.02, 0.02, 0.05, 0.05 μg/g, respectively. Standard reference materials (SRMs) such as oyster tissue, fish liver, and fish protein were chosen for method validation, and the results of five replicates were in accordance with certified values. Nine crabs, 4 lobsters, and 24 shrimps purchased from markets were analyzed for heavy metal concentrations in each tissue by this method. Mercury concentrations in all tissues of each sample were below LOQ. Cadmium, lead, and arsenic concentrations were different from sample to sample due to different sources and species. In lobsters, Pb was only detected in tomalley (N.D.~0.07 μg/g), and 94.30 ± 7.64% Cd was accumulated in tomalley (8.9~21.60 μg/g), which was significantly higher than that in other tissues. In carbs, 68.0 ± 27.40% Pb was accumulated in gills (0.14~0.54 μg/g), and 90.10 ± 5.4% Cd was accumulated in tomalley (0.02~10.62 μg/g), which was significantly higher than that in other tissues. In shrimps, Pd was only detected in cephalothorax (including viscera and head muscle), ranging from N.D.~0.07 μg/g, and Cd was only detected in cephalothorax, ranging from N.D.~0.76 μg/g. This research showed that higher Cd concentrations were found in tomalley of lobsters and crabs. We suggest that people should reduce intake of these high-risk tissues of crustaceans.