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篇名 PRELIMINARY INVESTIGATIONS OF GANODERMA AUSTRALE (SUBGEN. ELFVINGIA) IN TAIWAN
卷期 35:2
並列篇名 臺灣產南方靈芝(樹舌亞屬,靈芝科)之初步研究
作者 葉增勇陳瑞青
頁次 127-141
關鍵字 ScopusTSCISCIE
出刊日期 199006

中文摘要

自臺灣各地區濶葉樹採獲樹舌亞屬之標本12個,並自菌體組織獲得純培養。依據形態特徵,鑑 定本種爲南方靈芝Ganderma australe (Fr.) Pat. 。菌絲純培養經雜木層人工培養基栽培,生成 子實體。成功分離出菌株TAI-01之單孢系20個,由單孢系自身交配,顯示本種之交配系統爲異株 型、四極性。利用人工純培養菌株生長特性及單——雙核交配反應,本種尙可區分二羣。第一羣(包 括7個菌株)其最遒生長溫度是28-32°C,平均生長速率爲每日6.2 mm,單——雙核交配成親和反 應。第二羣(包括5個菌株)其最遍生長溫度是24-28°C >平均生長速率爲每日4.0 mm,單-雙 核交配成不親和反應。因此,作者認爲臺灣的南方靈芝已存在2個「雜交不孕性羣」。 由美國及荷蘭菌種中心購入之5株樹舌靈芝( G. lipsiense)參考菌株,其中ATCC 32585雙 核菌株分離自印度熱帶地區的濶葉樹,能和臺灣的南方靈芝TAI-01單孢系菌株成親和反應,並且 最適生長溫度及生長速率均類似於第一羣,推測本菌株可能和臺灣的南方靈芝同爲一種。 所有標本及純培養均貯存於國立臺灣大學植物學系,菌類實驗室。

英文摘要

Twelve specimens of Ganoderma, subgen. Elf vingia (Karst.) lmazeki were collected from hardwoods at several locations in Taiwan, and pure cultures were isolated. Based on morphology of the basidiocarps, all specimens were identified as Ganoderma australe (Fr.) Pat. 11onokaryotic cultures were obtainoo from basidiospores of artificially cultivated fruitbodies of the isolate TAI-01. The mating system was determined as heterothallic and tetrapolar. Using cultural studies and compatibility by dikaryon-monokaryon mating reactions, these isolates could be separated into two groups. Isolates of Group 1 (including 7 isolates) had an average growth rate of 6.2 mm/day at its optimum temperature range of 28-32°C. The di-mon matings were compatible. Isolates of Group 2 (including 5 isolates from the high altitude collection) had an average growth rate of 4.0 mm/day at its optimum temperature range of 24-28°C. The di-mon matings were incompatible. It is concluded that there are two "intersterility groups" of G. australe in Taiwan. Four monokaryotic isolates representing the mating types from Taiwanese collection of G. australe TAI-01 were mated with five isolates under the name of G. app/anatum (Pers.) Pat. (=G. lipsiense (Batsch) Atk.) obtained from ATCC or CBS. ATCC 32585, isolated from a tropical hardwood in India, was compatible with monokaryotic isolate T AI-01, and had a similar growth te1nperature requirement than Group 1 of G. australe from Taiwan. The other four isolates of G. lipsiense, ATCC 32586, CBS 175.30, 187.31, 250.61, were incompatible with G. australe TAI-01. ATCC 32585 of G. lipsiense is, thus, probably identical to G. australe.

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