本硏究以對低溫敏感的植物-綠豆(Vigna radiata L.,)爲材料。在黑暗中萌芽32小時的綠豆幼苗，經4°C處理不同時間後，分析其生長、粒線體活性及蛋白質合成能力等；其結果顯示下列各種現象皆受到抑制：㈠幼苗的生長，㈡粒線體的succinate dehydrogenease活性；㈢幼苗的蛋白質合成能力。但低溫處理會導致幼苗在室溫下呼吸速率增快，且幼苗的組織也受到傷害，因此組織內溶質的漏出物增加。在4°C低溫處理下所合成的35S-蛋白質，經SDS-膠體電泳分析及fluoro-graphy後，其中有13種蛋白質帶（protein band)是低溫誘導出的蛋白質（chilling induced proteins);其分子量是 110，88，78，69，58，48,38，27，22，19，14，12.5 及 10.5 KD，其 中88，69，58，48，38，14，12.5及10.5 KD是主要的低溫誘導蛋白質。這些蛋白質完全與綠豆的熱休克蛋白質不同。

Mungbean (Vigna radiata L., a chilling sensitive plant species, was used in this study. Seeds were germinated at 28°C in the dark for 32 h before cold treatment. The seedlings were chilled at 4°C for various durations. Growth, respiration rate, protein synthetic activity, cellular leakages and succinate dehj^drogenase activity in isolated mitochondria were then determined. Chilling at 4°C suppressed seedling growth, increased solute leakages, suppressed succinate dehydrogenase activity in isolated mitochondria, inhibited general protein synthesis and induced the synthesis of a set of proteins termed chilling-induced proteins^ The molecular weights of these chilling-induced proteins were 110，88，78，69，58, 48, 38, 27, 22，19，14，12.5 and 10.5 Kd，respectively and the proteins with molecular weights of 88, 69, 58, 48, 38, 14, 12.5 and 10^5 Kd were the major chilling-induced proteins of mungbean seedlings. These chilling-induced proteins were different from the heat shock proteins of mungbean seedlings.