Enzymatic isolation of protoplasts from young tissues of Chinese (Brassica pekinensis Rupr.) and Chinese chive 。 L.) has been studied. Different osmotica show variability in quantities of protoplasts. Chinese cabbage gives a good yield of protoplasts (10® cells/g tissue), but Chinese chive is poor (108 cell/g tissue). Spontaneous fusion of protoplasts occurs and its frequency increases with the prolongation of time. Induced fusion of protoplasts by PEG enhances the fusion frequency drastically (50%-70%). Vital staining with neutral red distinguishes between homokaryotic and heterokayrotic fusion. No heterokaryotic fusion occurring between protoplasts from these plants has been observed. INTRODUCTION