篇名 | 牛樟芝菌絲體及發酵萃取物對腸道α-葡萄糖苷酶抑制活性之研究 |
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卷期 | 53:4 |
並列篇名 | Inhibitory Effect on Intestinal α-Glucosidase by Extracts of Antrodia cinnamomea Mycelia and Cultural Filtrate Concentrate |
作者 | 黃贊勳 、 詹孟翰 、 蔡文琦 |
頁次 | 171-176 |
關鍵字 | α-葡萄糖苷酶抑制劑 、 牛樟芝菌絲體 、 牛樟芝發酵液 、 餐後高血糖 、 α-Glucosidase inhibitor 、 Antrodia cinnamomea mycelia 、 Antrodia cinnamomea cultural filtrate 、 Postprandial hyperglycemia 、 Scopus 、 TSCI |
出刊日期 | 201508 |
本研究探討以50%甲醇、80%甲醇及沸水萃取牛樟芝菌絲體及發酵液濃縮物,所得之萃取物對腸道α-葡萄 糖苷酶之抑制活性。試驗結果顯示牛樟芝的菌絲體及發酵萃取物都能抑制腸道α-葡萄糖苷酶的活性,且隨著 萃取物濃度越高,對腸道α-葡萄糖苷酶之抑制活性也越高。不論是菌絲體或發酵液皆以80%甲醇萃取物的抑 制活性最高,菌絲體萃取物之半抑制濃度為310.3 mg/mL,發酵萃取物之半抑制濃度為284.0 mg/mL。以菌絲體 80%甲醇萃取物進行酵素抑制動力學分析,結果顯示萃取物對腸道α-葡萄糖苷酶的抑制機制為非競爭型抑制 作用。研究結果顯示牛樟芝應可經由抑制α-葡萄糖苷酶活性,有效應用於餐後血糖值的控制。
The aim of this study was to evaluate the inhibitory activities of methanol and hot water extracts of Antrodia cinnamomea mycelia and cultural filtrate concentrate on intestinal α-glucosidase activity. There was a dose dependent percentage inhibition by the extracts against intestinal α-glucosidase. The enzymatic assay showed that 80% methanol extracts of both mycelia and cultural filtrate were more potent against intestinal α-glucosidase activity than 50% methanol extract and aqueous extract. IC50 values of 310.3 mg/mL and 284.0 mg/mL for mycelia and cultural filtrate were obtained, respectively. The enzyme kinetic study revealed that 80% methanol extracts inhibited intestinal α-glucosidase activity noncompetitively. The results of the study therefore indicate the potential of these extracts to manage postprandial hyperglycemia via inhibiting intestinal α-glucosidase activity.