山煙草富含抑制癌細胞生長之澳洲茄鹼類(solasonine)。本研究利用山煙草之 無菌苗為材料，誘導不定根及建立懸浮培養系統，並分析比較不定根、市售乾藥材及 植株各部位之澳洲茄鹼含量。研究結果顯示，山煙草無菌苗之根、莖、葉培植體培養 於含 1.0 mg.L-1 萘乙酸(1-naphthaleneacetic acid, NAA)組合 0.1 mg.L-1 6-苄胺基嘌呤 (6-benzylaminopurine, BAP)之MS基本培養基，間接經由癒傷組織誘導之不定根形成 率(87.5%400%)與形成數最高。山煙草不定根懸浮培養於不含生長調節劑之條件下， 增殖倍數可達28倍；每1週或2週繼代一次，5週後不定根增殖倍數分別可達100與 91倍。取初始鮮重為50、150、450 mg之不定根培植，體進行懸浮培養，顯示增殖倍 數隨初始重量增加而遞減，而累積生物量隨初始重量增加而遞增。利用高效液相層析 儀分析山煙草根、莖、葉、果實、市售乾藥材與不定根之澳洲茄鹼含量，以果實為含 量最高(25.8 mg.g-1 DW)，具顯著差異，其次依序為根部(21.4 mg.g-1 DW)、市售乾藥材 (19.4 mg.g-1 DW)、不定根(15.2 mg.g-1 DW)，而葉片與莖部並未偵測到。山煙草不定根 的澳洲茄鹼含量雖略低於果實、根部及市售乾藥材，但可透過懸浮培養進行量化生產， 未來可應用於生物反應器規模化增殖，以利於工業化生產。

Solanum verbascifolium L., belonging to the genus Solanum and the family Solanaceae, contains steroidal alkaloids with potent cytotoxicity of anti-cancer cells, and the roots are the major organ with active ingredients. In this study, i^ vitro plantlets of S. verbascifolium was used as plant materials for establishing suspension culture of adventitious roots and solasonine contents of different plant materials were compared to adventitious roots with HPLC. The combination of 1.0 mg-L"1 NAA and 0.1 mg-L"1 BAP is suitable for adventitious roots formation from explants of leaf, stem and root, in which the adventitious root formation rate is 100% and average root number is 14. With suspension culture, the proliferation rate of adventitious roots in liquid MS basal medium was up to 28-folds and increased to 100 and 91-folds with a subculture period of 1 or 2 weeks respectively after 5 weeks of culture. Different initial weights (50, 150 and 450 mg) of adventitious roots were used in suspension culture and the results showed that proliferation rate decreased with the initial weight, but the accumulated biomass increased in contrast. In 500 mg dry sample group, HPLC analysis showed that solasonine content of fruits (25.8 mg-g-1 DW) was significantly higher than that of roots (21.4 mg-g-1 DW), dry materials (19.4 mg-g-1 DW) and adventitious roots (15.2mg-g-1 DW), but solasonine content could not be detected in leaves and stem. Although the solasonine content of adventitious roots was slightly less than that of fruits, roots and dry materials, the adventitious roots with suspension culture could be used in mass production of plant materials and applied to bioreactor for industrial production.