本研究使用Afla Test-P Column及AFLAPREP M兩種商用免疫親和性層析管柱及螢光檢 測牛乳及乳粉中黄麴毒素3^，分析所得如下： (-)以Afla Test-P Column及榮光判讀機，分析人工添加0.5及l.Oppb黄麵毒素之脱脂牛乳， 其回收率各為84.4士7.0%及94.2±9.5%，其最低檢測限量為O.lppb，此法速簡，適例行性 大批檢體檢驗用。 ㈡以AFLAPREP M管柱及高效液相層析儀，分析人工添加0.1，0.5及l.Oppb黄麴毒素吣之脱脂 牛乳，其回收率各為84.7±2,7%，88.7±1.9%及89.2土4.2%，其最低檢測限量為0.05ppb， 此法較靈敏，適具高效液相層析儀實驗室例行性檢驗用。 ㈢以AFLAPREP M管柱方法，檢測牛乳及乳粉(乳粉檢驗前須沖泡成液狀乳)各25件，結果於3 件牛乳中分别檢出0.33，0.12及0.08ppb之黄麴毒素叫，乳粉則皆未檢出。

The method using immnno-affinity column clean-up and fluorescence measurement was evaluated for detection of aflatoxin M1 in milk and milk powder. Two kinds of commercial imrnunoaffinity columns used were Afla Test-P Column and AFLAPREP M. The results are shown below : (1) By using Afla Test-P Column and Fluorometer, the average recoveries of aflatoxin M 1 from defatted milk spiked at the levels of 0.5 and l.Oppb were 84.4±7.0% and 94.2±9.5%. The detection limit was O.lppb. Tbis method is easy and suitable for multi-sample treatment. (2)By using AFLAPREP M, the average recoveries of aflatoxin M1 from defatted milk spiked at the levels of 0.1, 0.5