A simple methodology for preparing synthetic multiple-interaction (SMI) chiral stationary phase (CSP) column is described in this report. The colimin was synthesized by an in situ derivati-zing procedure and can be easily prepared with a standard analytical HPLC set-up. The column is a no frills, low cost one, and can be tailored to various packing particle sizes and column lengths. The efficiency of the column is Wgh; over 5,000 plates per 10 cm. Applications on resolving some racemic drugs in plasma and formulation are illustrated.