最近臨場液態腔體電子顯微鏡的發明克服了傳統穿透式電子顯微鏡或掃描式電子顯微鏡難以觀察液態樣品的限制並已變成常規可得的技術，此技術能對於液體內所進行的物理現象或化學反應進行成像並且影像可具備奈米∼埃等級的空間解析度。受惠於此突破性技術而有重大進展的領域則涵蓋了材料科學與生物醫學。此處，我們使用臨場液態腔體穿透式電子顯微術來研究奈米氫氣泡的產生與其穩定性並區分群聚與單一奈米氫氣泡之間在穩定性上有顯著的不同，此外也發展定量單一奈米氣泡所含有氫氣氛子數量的方法，最後展示使用臨場液態腔體描式電子顯微術搭配自製的液體載台來觀察氣泡動態的實驗。

Recently, the invention of in situ liquid cell electron microscopy overcomes the limitation which liquid specimen is hard to be observed through conventional TEM (transmission electron microscope) or SEM (scanning electron microscope) and enables imaging physical phenomena or chemical reactions within liquid with nm ~ Å scale spatial resolution become routinely available. The signifi cant progress fi elds benefi t from this breakthrough technique could cover from material science to biomedical science. Here, we use in situ liquid cell TEM to investigate the generation and stability of hydrogen nanobubble and distinguish the different stabilities between a group of hydrogen nanobubbles and a single hydrogen nanobubble, moreover we also develop the method to quantify the hydrogen molecules amount within single nanobubble and fi nally we demonstrate the bubble dynamics via in situ liquid cell SEM with homemade liquid stage.