In recent years, the fashion industry introduced and promoted a number of new products that were labeled as eco-conscious, renewable, and sustainable. Unfortunately, the use of natural dyestuff for mass production could cause environmental issues, in terms of supply abundance. However, this concern could be averted with the use of pre-existing plant resources, namely, contained quantities of invasive plant species in Hawaii. In this research, dyes made from invasive species in Hawaii (Causurina equisetifolia, Clidemia hirta, Hibiscus rosa-sinensis, Lantana camara, Psidium cattleianum, and Spathodea campanulata) were tested against a commercial dye brand, Rit Liquid Dye in Navy Blue, using two American Association of Textile Chemists and Colorists (AATCC) standardized test methods for colorfastness and wrinkle recovery. A paper chromatography test was also used to determine if dye molecule size was a factor in how effectively the dye was absorbed into the samples. Results revealed that samples dyed with natural dyes had higher scores for colorfastness, and in general had a higher wrinkle-recovery score than Rit dyed samples. Chromatography also revealed that dyes made from Hibiscus rosa-sinensis and Spathodea campanulata had the highest retention factor (Rf) values of all the natural dyes, however, Rit brand dye had the highest Rf value of all the dyes. The results of this research reveal that natural dyes are a potential substitute for synthetic dyes for mass production. However, more research needs to be completed in order to keep the natural dyes vibrant, consistent and more colorfast between batches.