The authors have made an attempt to develop a practical method for the separation of bromelaiD and starch from pineapple stems. The bromelain was obtained cither by the acetone method or by the tannic acid method. Results showed that most bromelain was precipitated in the 40-50% acetone concentration, also that most bromelain was precipitated when 2-4 g of tannic acid were added per liter, under these condition the highest specific enzyme activity was obtained.