A new species, Gentiana pringlei (Gentianaceae) has been collected from Suru valley in Kargil district of Ladakh, Jammu and Kashmir. The new species shows morphological resemblance to G. loureiroi and G. napulifera, but differs from both in annual habit, absence of stolons, stem branched from base as well as from middle, three distinct veins on all the leaves, slightly keeled abaxially, purple flowers, ovate calyx lobes, sinus between calyx lobes truncate, corolla lobes oblong-elliptic, plicae bifid, shape and size of anthers and seeds small. The new species is described and illustrated here as Gentiana pringlei. In addition, the comparative morphological features with the sympatric species are tabulated.