Female produced sex pheromone of Maruca vitratacontaining three components had been reported from countries in tropical Asia and sub-Saharan Africa. (E,E)-10,12-hexadecadienal as a major component, (E,E)-10,12-hexadecadienol and E-10-hexadecenal as minor components blended in the ratio of 100:5:5 was found to attract M. vitratamale moths in few countries in West Africa. However, this blend was found to have less or no effectiveness in trapping M. vitratapopulation in Asia, which triggered the need to refine the M. vitratapheromone formulation. The isomers of major and minor pheromone compounds of M. vitratawere evaluated in this study. When subjected to Electroantennogram (EAG) analysis against male antennae, the isomer (Z,E)-10,12-hexadecadienal as a major component blended with the minor components (E,E)-10,12-hexadecadienol and (E)-10-hexadecenol in 100:10:5 was found to elicit better antennal response than the standard three component blend. GC-EAD profiles of the individual synthetic isomer components supported the EAG results. Additional experiments also confirmed the attraction of M. vitratapheromone compounds and their isomers along with host plant volatiles in EAG and wind-tunnel experiments. Field experiments in India and Cambodia confirmed the effectiveness of improved M. vitratapheromone lures in attracting the adult male moths in legume fields.