本研究以台灣彰化地區栽種之巨峰葡萄為原料經冷凍乾燥後，比較不同成熟度之凍乾巨峰葡萄（一期疏果、二期疏果以及成熟葡萄）其甲醇萃取物的抗氧化成份與抗氧化活性。利用體外如dpph、abts自由基與 H2O2清除試驗以及還原力等抗氧化試驗評估不同成熟度之凍乾葡萄其甲醇萃取物之抗氧化活性。試驗結果，以一期疏果葡萄甲醇萃取物有最高之總酚含量，二期疏果葡萄次之而成熟葡萄含量最低，分別為142.043 mg GAE/g dry extracts、68.121 mg GAE/g dry extracts及 11.615 mg GAE/g dry extracts。總黃嗣含量與總酣含量結果相 似，亦以一期疏果葡萄甲醇萃取物有最高之總黃酮含量（17.802 mg CTE/g dry extracts)、二期疏果葡萄（15.578 mg CTE/g dry extracts）次之而成熟葡萄含量（2.431 mg CTE/g dry extracts）最低。而花青素含量則隨著成熟度增 加花青素含量明顯增加，以成熟葡萄含量（1.962 "mo/e/g dry extracts）最高、二期疏果葡萄（0.853 "mo/e/g dry extracts）次之，一期疏果葡萄花青素含量(0.221 "mo/e/g dry extracts)最低。一期疏果葡萄甲醇萃取物之抗氧化 活性試驗除過氧化氫清除試驗外，在DPPH自由基（2,2-diphenyl-1-picrylhydrazyl radical）、ABTS(2,2-azinobis(3-ethylbenz-thiazoline)-6-sulfonicacid)自由基清除活性以及還原力試驗上之表現皆最佳。抗氧化活性與總酣和總黃酮含量呈現正相關性；綜合以上結果顯示凍乾之疏果巨峰葡萄的甲醇萃取物具有高含量的抗氧化成分（總酚與 總黃酮），且有最佳抗氧化活性，具有發展成為功能性食品的潛力，促進疏果葡萄之再利用。

The present study to assesses and compare the antioxidant properties of the methanol extracts of three growth stages, the first and second stage and the mature stage of lyophilized Kyoho grapes. The antioxidant potential of the extracts was assessed by employing different in vitro radical scavenging activities such as DPPH, ABTS free radicals and H2O2. The ability of reducing power was also evaluated. Results indicated the highest total phenolic content in methanol extracts was observed in the first growth stage of grape (142.043 mg GAE/g dry extracts), followed by the second stage (68.121 mg GAE/g dry extracts) and showed the lowest content (11.615 mg GAE/g dry extracts) in mature grapes. Total flavonoid content in methanol extracts from three growth stages of grapes showed the same trend as phenolics. It showed 17.802 mg CTE/g dry extracts, 15.578 mg CTE/g dry extracts, and 2.431 mg CTE/g dry extracts, respectively. However, anthocyanin content increased significantly with grape maturity. The anthocyanin was the highest in the mature grape (1.962μmole /g dry extracts), followed by the second growth stage of the grapes (0.853μmole /g dry extracts), and the lowest in the first growth stage of grapes (0.221μmole /g dry extracts). The first growth stage of grapes exhibited the highest antioxidant activity in all scavenging assays except for H2O2 scavenging assay. The antioxidant activity positively correlates with phenolic and flavonoid content. These results demonstrated that the methanol extracts in early growth stages of lyophilized grapes have excellent phytochemical content and antioxidant activities, indicating their potential applications for making functional food products.