本研究第一部分以南薑（Alpinia galanga, AG)地下莖之精油（AGO)及乙醇萃取物（AGE)與純物質1'-acetoxychavicol acetate (ACA)為實驗材料，探討其對3T3-L1前脂肪細胞分化及脂解作用之影響。細胞油紅染色結果顯示15-125 pg/mLAGO可抑制脂質的堆積，並向下調控脂肪細胞分化期間與脂質生合成相關基因之表現，如PPARy、C/EBPp、SREBP-1c、C/EBPa、GLUT4、LPL、aP2、CD36、ACC1 及FAS等。31-125 "g/mL AGO、7.8-31 pg/mL AGE及0.75ACA顯著降低肝素釋放脂蛋白脂解酶之活性；而31 pg/mL AGE則可促進脂解作用，提高脂肪三酸甘油脂解酶基因之表現，增加細胞之甘油釋放量。另以高脂飼料誘導肥胖之小鼠為模式，餵食 2〜10%之南薑凍乾粉末10週後，發現南薑粉末餵食組之體重較高脂組降低8.7〜15% ;體脂率下降1.4〜2.3% ;副 睪脂肪細胞半徑介於30-70 "m之細胞數目，由高脂組的59%下降至南薑餵食組的12〜15% ；血清中肝功能及血脂 參數亦顯著較高脂組為低。肝臟病理切片顯示南薑餵食組的脂質堆積較少，脂肪肝分數介於2.8〜3.9之間，較高脂組的4.8為低，另餵食南薑凍乾粉末後可顯著向上調控肝臟中促進脂質β氧化作用相關基因PPARa及CPT-1之表現量。綜合上述結果可知，南薑精油對抑制3T3-L1前脂肪細胞分化及脂質生合成的效果最好，動物實驗結果亦證實南薑凍乾粉末能減少脂質堆積、降低體重及體脂率，並改善因高脂飲食所誘發脂肪肝的潛力。

Obesity is a worldwide healthy problem that has attracted considerable attention. One of the aims of this study was to investigate the effects of pure compound (1'-acetoxychavicol acetate, ACA), essential oil (AGO) and ethanol extract (AGE) from Alpinina galanga (AG) rhizomes on 3T3-L1 preadipocytes differentiation and lipolysis in cell model. The results indicated that 15-125 μg/mL AGO could significantly inhibit the lipid accumulation determined by oil red O staining. The mRNA expressions of adipogenesis-related genes, including PPARγ, SREBP-1c, C/EBPα, GLUT4, LPL, CD36, aP2, ACC1 and FAS were significantly down–regulated by 31-125 μg/mL AGO during the 3T3-L1 preadipocyte differentiation. Moreover, we also investigated the prevention effect of fat accumulation from Alpinia galanga freeze-dried powders (FA) in high fat diet (HFD)-induced obese BALB/c mice. The obese mice were oral administrated by 2, 4 and 10% FA (LFA, MFA and HFA respectively) powder for 10 weeks. The results showed that the decreases of body weight and body fat ratio of LFA (8.7 and 2.3%), MFA (9.1 and 1.6%) and HFA (15.0 and 1.4%) groups were raised ( p < 0.05). Moreover, administrations of FA could reduce the serum triglyceride, total cholesterol, aspartate aminotransferase, alanine aminotransferase levels. The fatty liver in FA groups were ameliorated and the expressions of lipid β-oxidation-related genes PPARα and CPT-1 were up-regulated by MFA and HFA in the liver ( p < 0.05). These results provide the evidences that Alpinina galanga rhizomes can inhibit the 3T3-L1 preadipocyte adipogenesis and lower the risk of obesity in obese BALB/c mice.