蜂蜜摻偽是食品摻假常見的議題，然而目前國際公認且通用的檢測蜂蜜摻偽技術：穩定碳同位素比值分析，除需依賴昂貴的質譜儀且檢測程序繁瑣及耗時，更無法作為常規篩檢用途。本研究將26件市售調和式蜂蜜、48件純龍眼蜜、23件純荔枝蜜進行糖度、酸鹼值、導電度、顏色強度、濁度、脯胺酸含量以及葡萄糖酸含量等七種物理化學性質分析，並從試驗數據中檢討出各項性質用以區分純蜂蜜與調和式蜂蜜之閥值，隨後透過正確辨別率計算來決定適用的摻偽蜂蜜快速篩檢指標。試驗結果顯示糖度、酸鹼值與顏色強度對於篩檢摻偽蜂蜜不適用因為出現偽陰性之檢測結果；而導電度、濁度、脯胺酸含量以及葡萄糖酸含量等四種性質具有潛力可作為摻偽蜂蜜之初步快篩指標。其中，樣品導電度值小於170 μS/cm者、或濁度值小於0.058者、或脯胺酸含量小於180 mg/L者、或葡萄糖酸含量小於93 nM者，皆被篩選成為可疑樣品。根據上述檢測結果先將陰性檢體大幅排除後，剩餘陽性檢體再進行穩定碳同位素比值分析，便能達到成本降低、省時便捷之快篩目標。

Honey adulteration is a topic issue in the field of food authenticity. Although stable carbon isotope ratio analysis is commonly recognized to be the international standard procedure for honey adulteration, it is still too costly, time consuming, and laborious which is inapplicable to routine screening purposes. In the present work, 26 commercial blended honeys, 48 pure Longan honeys, and 23 pure Litchi honeys were collected to analyze their physicochemical properties including sugar content, pH value, electrical conductivity, color intensity, turbidity, proline content, and gluconate content. The threshold of each physicochemical property for discriminating between pure honeys and blended honeys was then designated according to the experimental data obtained above. After calculating the discrimination ratio, the applicable rapid screening indexes were selected for honey adulteration. It was revealed that sugar content, pH, and color intensity were not applicable to screen honey adulteration due to false negative results were found. Contrarily, electrical conductivity, turbidity, proline content, and gluconate content were proven to be the potentially applicable preliminary rapid screening indexes for honey adulteration. The suggested thresholds of electrical conductivity, turbidity, proline content, and gluconate content were set to be 170 μS/cm, 0.058, 180 mg/L, and 93 nM, respectively. Once any of the four rapid screening indexes was determined less than the corresponding thresholds, the sample was screened out as suspicious sample. Therefore, the negative samples could be substantially screened out accordingly, the remained positive samples were then verified by the stable carbon isotope ratio analysis to realize an ideal rapid screening method with the features of cost-effectiveness, easy-to-use, and time-saving.