幽門螺旋桿菌的診斷方式有分侵入性及非侵性檢查，其中侵入性檢查中有內視鏡檢查，藉由胃部組織切片，從病理組織變化，快速尿素酶檢測及細菌培養鑑定[ 尿素酶 (urease)、觸酶 (catalase) 與氧化酶 (oxidase)] 等試驗鑑定之結果，確認為幽門螺旋桿菌得知，其中以細菌培養最困難。臨床上，不管三合一療法或四合一療法，都有一定治療失敗的比率。因此，藉由提升幽門螺旋桿菌培養分離率，才能探究此菌株抗生素敏感性，輔助臨床除菌用藥並提升根除率。本研究探討不同培養基對於幽門螺旋桿菌分離率的差異分析。本研究分別選擇三種培養基(plates)，分別為CDC plates (CDC Anareobic agar plate); EYE plates (EYE anaerobic agar)及BAP plates (Blood agar plate)；選擇採檢後24 小時內及72 小時內完成幽門螺旋桿菌培養差異比較，期望能提升幽門螺旋桿菌之高分離率。結果顯示，檢體採檢24 小時內完成幽門螺旋桿菌培養分離率結果為CDC plates 73%，EYE plates 53%，BAP plates 100%。檢體採檢72小時內完成幽門螺旋桿菌培養分離率結果為CDC plates 37.5%，EYE plates 13% 及BAP plates100%。臨床上，胃部檢體採檢後若未能立即進行幽門螺旋桿菌培養，會降低篩出幽門螺旋桿菌的機率，結果顯示，BAP plates 於檢體採檢24 小時內及72 小時內相較於CDC plates 及EYE plates 均具有高分離率(100%)，不受檢體採檢後72 小時才進行幽門螺旋桿菌培養而影響分離率。根據臺灣幽門螺旋菌治療共識，若患者於二線經驗性除菌治療後未能成功除菌，建議進行藥物敏感性的測驗以之訂定治療方案，高篩選幽門螺旋桿菌分離率將有助於菌之抗藥性分析及提升臨床之精準用藥。

Helicobacter pylori infection can be diagnosed by non-invasive techniques and by invasive techniques via endoscopy examination and biopsy (e.g. histological examination, rapid urease test and culture with urease, catalase and oxidase base culture plate) and the most challenging part is culture due to technical difficulties. Although combination treatment of triple therapy and quadruple therapy are effective for eradicating Helicobacter pylori, a significant portion of patients failed the eradication treatment. Furthermore, we could investigate susceptibility of each strain and increase eradication rate by improving Helicobacter pylori isolation rate. This study focuses on the different analysis from distinct culture plate for Helicobacter pylori. Three types of plate were chosen, CDC plates with blood agar, EYE plates with Helicobacter pylori selective agar and BAP plates with blood agar. The endpoints of isolation rate were 24 hours and 72 hours after cultivation for determine better isolation rate. The result yielded that isolation rate of 73% with CDC plates, 53% with EYE plate, and 100% with BAP plates at 24 hours after cultivation. Meanwhile, at 72 hours after cultivation, 37.5%, 13% and 100% of CDC plates, EYE plates and BAP plates respectively. BAP plates undoubtedly obtained better result than CDC plates and EYE plates. Better isolation rate of Helicobacter certainly aided analysis of antibiotic susceptibility and resistance thus improved the accuracy of eradication regimen in clinical practice.