Stixis villiflora (Resedaceae), a new species from Mengla, Yunnan, China, is described and illustrated. It is compared with four morphologically similar species S. philippinensis, S. suaveolens, S. ovata subsp. fasciculata and S. scandens. It differs from the preceding species by both leaf surfaces being pubescent and full of pustules, stamens 14–18, filaments lower half pubescent, upper half sparsely pubescent or glabrous, androgynophores ca. 1 mm long, lower half glabrous, upper half tomentose, gynophores and ovary with densely white hairs. A complete morphological description of Stixis villiflora is provided, together with photographs, a conservation assessment, and a diagnostic key to 10 species and 1 subspecies of Stixis from Asia.