本研究主要探討大豆油以氫氧化鈉催化甘油解法（SHCG）與脂解酶催化甘油解法（LCG）製備雙甘油酯（diacylglycerol, DAG）。將製備之粗DAG以管柱區分進行純化，得到富含1,3-DAG。結果顯示，SHCG最適條件是將大豆油加入0.5莫耳比（甘油/油）之甘油於50°C下在氫氧化鈉催化反應2小時，並以丙酮作為溶劑。DAG 的產量可達50.27%。上述條件，若以乙醇取代丙酮，其DAG的產量可達32.28%。LCG最適條件是將大豆油加入10%之脂肪酶，並加入0.5莫耳比（甘油/油）之甘油，於50°C下反應8小時，DAG的產量可達44.55%。粗DAG 經由管柱純化之後，SHCG製備之1,3-DAG含量為43.09%，此結果表示約有90%的DAG是1,3-DAG。LCG製備之 1,3-DAG含量則為31.18%，此結果表示約有70%之DAG為1,3-DAG。

This study was to investigate the diacylglycerol (DAG) prepared from soybean oil with sodium hydroxide catalyzed glycerolysis (SHCG) and lipase catalyzed glycerolysis (LCG). The crude DAG was purified by the column fractionation to obtain 1,3-DAG-enriched. The results showed that the optimal condition of SHCG was: glycerol/oil ratio of 0.5 mol, reaction temperature of 50°C, sodium hydroxide catalyzed reaction time of 2 hrs and acetone as solvent. The yield of DAG could reach up to 50.27% by using this reaction condition. The yield of DAG could reach 32.28% when acetone was substituted by ethanol under this condition. In addition, the optimal condition of LCG was: glycerol/oil ratio of 0.5 mol, reaction temperature of 50°C and reaction time of 8 hrs. The yield of DAG was maximized to 44.55% when DAG was prepared by this condition. After column purification, the 1,3-DAG content of the crude DAG prepared by SHCG was 43.09%. This result indicated that the content of 1,3-DAG was about 90% in purified DAG. On the other hand, the 1,3-DAG content of the crude DAG prepared by LCG was 31.18%. This result indicated that the content of 1,3-DAG was around 70% in purified DAG.