水產膠原蛋白逐漸受到消費者的喜愛，目前水產膠原蛋白主要來源為魚類，而在無脊椎動物之膠原蛋白上的研究較為稀少，因此本研究探討黑赤星海參(Holothuria cinerascens)與吳郭魚(Oreochromis niloticus)皮的組成分、膠原蛋白結構及吸濕與保濕性功效，評估其作為化妝品原料之潛力。經SDS-PAGE分析，黑赤星海參與吳郭魚皮為I型膠原蛋白，分別由(α1)3鏈及(α1)2α2鏈所組成，紫外光譜結果顯示黑赤星海參與吳郭魚皮分別在236.5nm及235.5nm有最大之吸收波峰。膠原蛋白吸濕性在RH43%及81%時，黑赤星海參較吳郭魚皮高出約5%;膠原蛋白保濕性在RH43%下，二物種和甘油皆無顯著差異；在RH81%的情況下，二物種的保濕性皆無顯著差異，但遠高於甘油。整體來說，黑赤星海參的膠原蛋白功效性方面較吳郭魚皮好，且保濕性遠高於甘油，為具有發展潛力之化妝品原料。

Recently, consumers have preferred using aquatic collagens. The main source of aquatic collagens come from fish, and the researches on collagens of sea cucumber are rare. Therefore, this study explored and compared the constituents of hydrolysates, the amino acid composition, structure, moisture absorption and retention properties of the collagens from Holothuria cinerascens and Oreochromis niloticus skin, and evaluated their potentials as cosmetic materials. Based on results of SDS-PAGE, the collagens from H. cinerascens and O. niloticus skin were type I collagens and mainly composed of (α1)3 and (α1)2α2 chains. Results of UV spectrometry showed that collagens from H. cinerascens and O. niloticus skin had a maximum absorption peak at 236.5 nm and 235.5 nm, respectively. In cases of the relative humidity (RH) at 43% and 81%, the collagens isolated from H. cinerascens had about 5% higher moisture absorption rate than collagens from O. niloticus skin. However, moisture retention rate had no significant difference among the collagens from two samples and the glycerol at RH 43%, but both collagen samples were significant better than the glycerol at RH 81%. In general, the collagens from the H. cinerascens had better efficacy on moisturizing than those of O. niloticus skin. Results suggest that collagens from the H. cinerascens can be potential moisturizing materials for cosmetic products.