食品熱殺菌加工程序中必須滿足對微生物殺菌安全之要求，同時也能避免過多營養成份降解，因此殺菌溫度與時間成為熱殺菌過程中反應動力學主要參數。目前已有相當多文獻報導利用微生物方法或是與特定化學成分進行熱致死時間 (TDT) 之動力學研究，但因為材料成本、試驗步驟、量化與分析方式均相當昂貴與繁瑣，而且無法適用於高過100℃ 之高溫條件，雖然可建立熱致死動力學數據，但作為時間與溫度累積其對殺菌效能指標成分則有應用上的難度。本研究提出一種乳清蛋白凝膠中M-2 (4-Hydroxy-5-methyl-3(2H)-furanone)做為化學標記，其特性為可於實驗室簡便且精確製備其標記成分含量，將放置於外徑為6.35公厘、長度為10公分之不銹鋼管中，改變不同殺菌溫度範圍為110~130℃ ，利用HPLC定量分析方法，建立M-2動力學參數。試驗結果發現，M-2於殺菌溫度110、120、130℃ 下經反應一段時間後，其濃度將達平穩狀態而不再增加，其反應動力學模型為一階反應，M-2反應終點濃度M∞分別為967、881、913 ppm，反應速率k值則分別為0.104、0.161、0.310 ( ppm/min)，對應其反應活化能 (Ea) 為17.35 kcal/mol K。本研究建立M-2反應動力學參數條件以及分析方法，可作為相關研究人員進行等效熱殺菌溫度與時間試驗之標準測試材料參考用。

Food sterilize by the use of heat treatment process needs to satisfy the safety requirements for microorganism sterilizing as well as avoiding deterioration of the product’s nutritional content. Therefore it is important to optimize the temperature and duration of the sterilizing process. Currently there is plenty of existing literature that studied the dynamics of Thermal Death Time as part of the heat treatment sterilizing process, however, due to the material cost, experimental procedures, quantifying and analysis method are complicated and costly, and is unsuitable for an environment over 100oC, it is difficult to act as an indicator and application procedure for Temperature and Time integrator sterilizing efficiency, even though the thermal dynamics data can be constructed. This research suggests M-2 (4-hydroxy-5-methyl-3(2H)-furanone) to act as a chemical marker. Its properties allow simple and precise marking of the content level. Putting it in a steel tube with outer diameter of 6.35 mm length of 10 cm, changing the sterilsing temperature from 110~130oC, utilizing HPCL fixed volume analysis method, to construct M-2 dynamic pramaters. The results showed that the concdentration of M-2 stabilizes and cease to increase after sterilsing at 110, 120, 130oC for a time period. The mathmatical dynamics model used is the First-order reaction with the M-2 marker end concentration to be 967, 881, 913 ppm respectively to the temperature. Reaction speed, the k value were 0.1, 0.16, 0.31 ( ppm/min) respectively. The activation energy (Ea) was 17.35 kcal/mol K. The dynamic parameter characteristics analysis used in this particular study can be used as standards for the different temperatures and sterilizing durations.