Lactobacillus paracasei subsp. paracasei NTU 101 (NTU 101) 為具有多種功效的保健食品菌株，但目前尚未有發酵槽生產的相關研究。本研究以2.0 L發酵槽探討氧氣及pH值對於NTU 101生長動力學之影響。結果顯示通入N2 進行培養可得較高細胞密度 ((2.36 ± 0.21) × 109 CFU/mL) 及比生產率 ((1.97 ± 0.18) × 108 CFU/mL/h)。而控制在pH 6.0下進行培養可得最高的比生長速率 (0.61 h-1，p < 0.05)，並使得發酵時間縮短至9小時 (縮短為0.56倍)。相較於初始條件，在最適化條件下NTU 101的比生產率提升了12.89倍。以10% (w/v) 豆寶二號豆漿粉作為替代性培養基，可顯著提升1.53倍單位成本產率。RT-qPCR分析結果顯示通入N2 培養會降低DnaJ、DnaK、sHsp和GroES基因的表現量，控制於最適pH會再進一步降低DnaJ、DnaK及GroES的表現，顯示NTU 101的生長限制和這三個基因的表現量有關。由於有氧環境、較高或較低pH值環境下表現Csp、DnaJ、DnaK、sHsp和GroES均參與蛋白質的摺疊修復機制，因此逆境所造成的蛋白質錯誤折疊可能是本實驗中主要限制NTU 101生長的因素，未來可利用這些基因表現量作為其生理狀態的指標，進一步探討逆境處理與NTU 101存活能力間的關聯性。

Despite Lactobacillus paracasei subsp. paracasei NTU 101 (NTU 101) is a well-known multi-functional health food strain, study for its bioreactor production is still absent. Effects of oxygen level and pH on the growth dynamics of NTU 101 were investigated in this study. Results showed that the highest specific growth (0.61 h-1, p < 0.05) rate was achieved by nitrogen sparging and consequently reduced fermentation time to 9 hours (0.56 fold). The optimized fermentation condition for NTU 101 raised 12.89 fold of initial specific productivity. The unit productivity could be significantly increased by 1.53 fold by using alternative media- 10% (w/v) soya milk powder under optimized condition. RT-qPCR analysis revealed that nitrogen sparging down-regulated the expression level of DnaJ, DnaK, sHsp and GroES genes. Introducing of optimized culture pH further reduced expression levels of DnaJ, DnaK and GroES genes, which indicates that the growth of NTU 101 was related to these three genes. The elevated expression levels of Csp, DnaJ, DnaK, sHsp and GroES genes under oxidative, high pH and low pH conditions also suggested that protein misfolding may be the major limit factor to NTU101 growth since these genes were all participated in protein misfold and refold pathways. The relationship between stress pretreatment and survival ability of NTU 101 cells will be investigated with expression level analysis of these genes in the future.