層光螢光顯微鏡可提供高三維空間分辨率、高訊噪比、快速成像採集速率以及較低的光毒性和光損傷效應，這些特性使其在生物醫學領域中至關重要，並涵蓋了廣泛的應用範圍，從單個細胞的動態學到大型生物體內發育動態的高解析度成像。在這篇簡述中，我們提供了層光螢光顯微鏡的歷史發展及其變體和改進的討論，以及層光螢光顯微鏡的最新技術進展及其在細胞、單分子偵測和大型組織的應用。

Light sheet fluorescence microscopy provides high three-dimensional spatial resolution, high signal-to-noise ratio, fast imaging acquisition rate, and low phototoxicity and photodamage effects. The characteristics are important in the biomedical field from the dynamics of single cells to developmental dynamics in large organisms. In this article, we provide a discussion on the historical development of light sheet fluorescence microscopy, the latest technological advances, and its applications in cells, single molecule detection and large tissues.