本研究中使用可撓矽膠管延長大氣電漿束進行實驗，並利用增強電荷耦合元件相機(intensified CCD camera, ICCD camera)、光學放射光譜儀(optical emission spectroscopy, OES)及示波器測量不同矽膠管長度的大氣電漿束的電漿強度、OH及O自由基強度分布、電漿的放射光譜及電漿電能效率等生物醫學應用時重要的參數。研究結果顯示，電漿強度最大的位置隨矽膠管長增加而向管口靠近，而電漿與自由基的強度及電漿電能效率皆隨矽膠管增長而變化。第二部分研究測試了延長型大氣電漿在不同工作距離的抑菌效果，結果顯示當工作距離大於等於2.0 cm時抗菌效果最佳，抗菌區域為實心圓；而當工作距離小於等於1.0 cm時，抗菌區域則出現環狀結構。為了解實心圓與環狀抗菌區域的形成原因，我們進而利用煙霧實驗觀察氦氣流流向，並以ICCD相機記錄當工作距離為1.0 cm與2.5 cm時，電漿、OH自由基與O自由基在石英玻璃板上的分布情形。煙霧實驗與ICCD影像對照結果顯示氦氣流影響了OH自由基的分布，而OH自由基為主要抗菌物質，因而造成不同型態的抗菌區域。

Recently, atmospheric plasma technology has been found to have a huge potential and bright future in plasma medicine. In practice, the flexibility and extendibility of plasma jets are critical in many of the bio-medical applications. In this study, silicone tubes were used to extend the length of plume. Silicone was chosen because it is a non-toxic, biocompatible, highly elastic material. A customized atmospheric pressure plasma jet (APPJ) equipped with unipolar pulse power supply was used to generate He plasma for the present study. The plumes were analyzed by OES as functions of tube length and working distance in order to realize the distribution of OH and O radicals. The results show that the intensity ratio of OH/He and O/He in the discharge decreased with the increase of silicone tube length. In the study of working distance, With the increase of tube length, the decrease of OH intensity was found to be more significant than O’s. With the increase of working distance, the combination of OH+O intensities showed a peak appearing at 1.5-2 cm. Accordingly, the bactericidal efficiency was influenced. In order to realize the formation of bactericidal circle pattern, ICCD camera and smoke flow were used. It can be concluded that the distribution of OH radical is the main reason that caused dual circles.