本研究是利用模擬移動床連續分離純化蟲草素(cordycepin) 達純度60%~90%。研究中使用的模擬移動床具備6 支ID10 × L250 mm 的C18 層析管柱，並基於三角理論預測模擬移動床的操作條件。結果顯示，當模擬移動床進出口流速設定為移動相(Desorbent) 6.0 mL/min；進料(Feed)0.6 mL/min；萃出液(Extract) 2.5 mL/min；萃餘液(Raffinate) 4.1 mL/min，切換時間為3 分10 秒時，可獲得最佳純化條件，純度可由萃取物中的4.06％提高到65.67%。蟲草素具有抗發炎、抗腫瘤及抗衰老等功效。因此在生技、醫藥及美妝產品的應用上，預期能有很大的發展潛力。根據食品藥物管理署公告，北蟲草萃取物每日使用量以蟲草素計算，不得超過3.6 毫克，但若服用量太少則無顯著效果。因此，若是能將蟲草素提純，就能輕易控制使用劑量。模擬移動床是一種連續式的層析裝置，能提高蟲草素的純度，去除多餘雜質。如能取得較高純度蟲草素的原料能夠幫助生產者將成本降低及進行產品成分開發。此設備可解決傳統純化設備僅能批次式進行及消耗許多溶劑和耗材的問題。所使用之溶劑不像傳統方法使用大量有機溶劑，而是容易取得的水與乙醇，對環境不會額外產生污染。另外模擬移動床使用電腦控制並且能夠24 小時連續運轉，可大幅度的降低人力成本。未來亦能運用在其他天然物的純化應用。

In this study, a simulated moving bed was used to continuously separate and purify cordycepin to a purity of 60% to 90%. The simulated moving bed used in the study is equipped with 6 chromatography columns (C18, ID10 × L250 mm), and the operating conditions of the simulated moving bed are predicted based on the triangle theory. The results show that when the inlet and outlet flow rate of the simulated moving bed is set to the Desorbent 6.0 mL/min; Feed 0.6 mL/min; Extract 2.5 mL/min; Raffinate 4.1 mL/min, when the switching time is 3 minutes and 10 seconds, the best purification conditions can be obtained, and the purity can be increased from 4.06% in the extract to 65.67%. Cordycepin effects is anti-inflammatory, anti-tumor and anti-aging et al. Therefore, the application of biotechnology, medicine and beauty products, it is expected to have great development potential. According to the Food and Drug Administration's announcement, the daily usage of Cordyceps militaris extract is calculated based on cordycepin, which should not exceed 3.6 mg, but if the amount is too small, it will not have a significant effect. Therefore, if the cordycepin can be purified, the dosage can be easily controlled. Simulated moving bed is a continuous chromatography device that can improve the purity of cordycepin and remove excess impurities. The raw materials with higher purity cordycepin can help producers reduce costs and develop product ingredients. This equipment can solve the problem that traditional purification equipment can only perform in batches and consumes many solvents and consumables. The solvent used is water and ethanol that are easily available different from the traditional method using a large amount of organic solvents which will not cause additional pollution to the environment. In addition, the simulated moving bed is controlled by a computer and can run continuously for 24 hours, which can greatly reduce labor costs. It can also be used in the purification of other natural materials in the future.