細胞的排列對於許多的細胞活性相當地重要，因此需要一個能夠誘導細胞排列生長的細胞養殖系統，當此類細胞壞死或是損壞時，能夠適時地進行修復，像是神經細胞、牙髓細胞等。在這份文章中，我們利用由Parylene C此聚合物所製成的具有排列性孔洞的高分子支架，來模擬細胞在體內的排列生長情形。由於有好的生物相容性以及生物穩定性，Parylene C被廣泛地運用在侵入式的醫療設備當中。這個具有排列性孔洞的高分子支架是透過化學氣相沉積（chemical vapor deposition, CVD）的程序所製成。將含有羧甲基纖維素（carboxymethyl cellulose, CMC）的水溶液沿著一個特定方向結凍並放入化學氣相沉積的反應室中，在高溫下，欲沉積的聚合物單體與昇華的水氣間就會產生質傳的交互作用並得到產物。後續再利用電子掃描顯微鏡（SEM）以及3D雷射共軛交顯微鏡（3D laser confocal microscope）等儀器進行材料的觀察。本實驗所採用的細胞為小鼠胚胎成纖維細胞（3T3 cell）以及人類牙髓幹細胞（hDPSCs），將所選細胞養殖於材料上後並利用螢光顯微鏡進行細胞觀察，可以明顯觀察到細胞呈排列生長，後續再利用細胞活性的測量方法量測細胞於材料上的活性。由於目前尚無醫療方法可以有效地治療蛀牙，因此本技術具有相當大的潛力可以做為蛀牙治療的手段之一，甚至有機會可以在許多組織工程的應用上利用本技術作為主要的核心技術。

Cell alignment is a vital component in various cell activities, therefore, a culture system that could induce cell alignment would be of great importance. In this report, we simulated the oriented cell alignment in the body by culturing the cells on an aligned scaffold made of Parylene C, which is a material that is widely used for implantable medical devices due to its biocompatibility and biostability. The aligned scaffold was fabricated by using chemical vapor deposition (CVD) process. In the process, a water solution containing carboxymethyl cellulose (CMC) was slowly frozen before being inserted into the reaction chamber, where a mass transfer between the deposited monomer and sublimating vapor will occur. The scaffold obtained was then observed with scanning electron microscopy, where the aligned structures were clearly seen. Furthermore, 3T3 cells and human dental pulp stem cells (hDPSCs) were cultured on top of the aligned material and when seen through fluorescence microscope, the alignment of c ells were also distinctly observed. This shows that the fabricated Parylene C scaffold could facilitate cell alignment and might make a contribute to the tissue engineering application such as the tooth decay treatment.