Cordyline [Cordyline fruticosa (L.) A. Chev.] has been widely used as potted plants and for landscaping in subtropical and tropical areas. However, in Taiwan, almost all cultivars have been imported from overseas due to lack of information on flowering regulation, pollination technique, and seed propagation. A spray of 5000 mg．L^(-1) GA_3 on leaves of 12 cultivars was conducted in April, 2012, September, 2013, October, 2019, and April, 2020, respectively. Results showed that flowering occurred 8-14 weeks after treatments in September and October for most cultivars, including 'Fire Fountain', 'Plumdinger', 'Red Sister', 'White Edge', 'Cooperi', 'John Klass', 'Thelma', 'Cameroon', 'Cointreau', 'Dolly', 'Miss Andrea', and 'Moonlight'. The pollen of 'Red Sister' and 'White Edge' was collected from natural flowering plants in winter. The highest pollen germination rate was measured in 15% sucrose treatment when cultured in B&K medium with 0% to 20% sucrose. All pollen of 'Tricolor' germinated when collected at anthesis, whereas no pollen germinated one day before or after anthesis. Tissue section revealed that pollen germinated and pollen tubes reached the base of the style but not the ovary in self-pollinated flowers of 'Red Sister', indicating gametophytic incompatibility. Physical scarification increased germination rate and reduced germination time of hybrid seeds from 'Fire Fountain' crossed with 'White Edge'.