薏仁麩皮(adlay bran, AB)具抑癌、抗發炎、改善代謝症候群、調節內分泌等效，酚類、多甲氧基類黃酮(polymethoxyflavones, PMFs)與其特有的薏苡內醯胺(coixspirolactam)是重要活性成分，然目前未有針對上述物質製備萃取物的優化程序。本研究發現AB乙醇萃取物(ethanolic extract, ABE)萃取率、總多酚、總類黃酮主要受萃取溫度與乙醇濃度影響，藉反應曲面法(response surface methodology, RSM)獲得以8倍AB重量體積之55%乙醇於50oC萃取120分鐘之最適化條件進行樣品製備，高效液相層析(high-performance liquid chromatography, HPLC)分析顯示，酚類以對羥基苯甲酸(p-hydroxybenzoic acid, 11.86 μg/g)、香草醛(vanillin, 6.57 μg/g)與阿魏酸(ferulic acid, 6.36 μg/g)較豐富，HPLC串聯質譜(HPLC-MS)可明確比對coixspirolactam E、3,5,7,4'-tetramethoxyflavone、3,5,7,3',4'-pentamethoxyflavone、3,5,6,7,8,3',4'-heptamethoxyflavone、橘皮素(tangererin)與川陳皮素(nobiletin)等PMFs，上述試驗結果將可運用在AB活性萃取物新產品開發與品質管制。

Adlay bran (AB) possesses physiological functions on inhibiting cancer, anti-inflammation, improving metabolic syndromes, and modulation on endocrine. Phenolic compounds, polymethoxyflavones (PMFs), and unique coixspirolactams were regarded as important active components in AB. However, optimized extracting procedure for above ingredients is lacking. In the present study, yield, total polyphenol, and total flavonoids contents in AB ethanolic extract (ABE) were founded to be affected mainly by temperature and concentration of ethanol(aq). According to response surface methodology (RSM) evaluation, the optimized extracting condition of ABE was under 50°C with 8-folds of 55% ethanol(aq) for 120 min. ABE made by above procedures was analyzed on a high-performance liquid chromatography (HPLC) system; and p-hydroxybenzoic acid (11.86 μg/g), vanillin (6.57 μg/g), and ferulic acid (6.36 μg/g) were relatively abundant in ABE. Moreover, coixspirolactam E and PMFs, including 3,5,7,4'-tetramethoxyflavone, 3,5,7,3',4'-pentamethoxyflavone, 3,5,6,7,8,3',4'-heptamethoxyflavone, tangererin, and nobiletin, were clearly identified by HPLC-mass spectrum (HPLC-MS) platform. The results would be used for product development and quality control of ABE.