篇名 | Solid-State Fermentation of Pectinase and Amylase from Bread Waste Using Bacillus Strain (BIOSMNF02) |
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卷期 | 62:1 |
作者 | Anushka Satpathy 、 Farheen Fatima 、 Akhil Kumar Sen 、 Koel Mukherjee 、 Vinod Kumar Nigam |
頁次 | 012-020 |
關鍵字 | Amylase 、 Bread-waste 、 Pectinase 、 Enzyme Assay 、 DNS method 、 Moisture percentage 、 Solid state fermentation 、 Scopus 、 TSCI |
出刊日期 | 202403 |
DOI | 10.6578/TJACFS.202403_62(1).0002 |
Despite modern-day technology, food waste is still a major concern. Bread is one of the major consumable foods in Asian countries and has also been observed as waste in various institutions. Fermentation is one of the most socioeconomic methods of utilizing various types of raw materials including biomass as waste. The present work highlights the production of enzymes pectinase and amylase using a Bacillus strain (BIOSMNF02) and beard waste as raw material under minimal water content. Among all the physio-chemical parameters attempted in the minimum water content, the optimum result was obtained in a medium having a moisture level of 1:2.25 (w/v) at pH 7, with the inoculum percentage as 4% (w/v). The optimum fermentation period for the maximum pectinase and amylase production was found to be on the 5th day of culturing condition and the 10th day of fermentation respectively. The maximum activity of enzymes was found to be at 37℃, pH 7, and substrate concentration of 200 μg/mL of pectin and 300 μg/mL of starch for pectinase and amylase respectively. The selected bacterium, Bacillus strain (BIOSMNF02) was isolated for its pectinase production capability, but it also showed the synthesis of amylase that offered novelty in the present study.