本研究開發逆相高效液相層析法同時檢測光葉苦蕒菜(Ixeris
chinensis)中6種Sesquiterpene Lactones：3β-[3-(4-hydroxyphenyl)acetyl-β-
D-glucopyranosyloxy]-8β-hydroxy-4(15),10(14)11(13)-guaiatrien-12,6-olide
(AR1); 3β-[3-(4-hydroxyphenyl)acetyl-β-D-glucopyranosyloxy]-4(15),10(14),
11(13)-guaiatrien-12,6-olide (AR2); 3β-(β-D-glucopyranosyloxy)-8β-(4-
hydroxyphenyl)acetoxy-4(15),10(14),11(13)-guaiatrien-12,6-olide (AR3)
3β-(β-D-glucopyranosyloxy)-8β-(2-hydroxy-3-methylbutanoyloxy)acetoxy-
4(15),10(14)11(13)-guaiatrien-12,6-olide (AR4); 3β-(β-D-glucopyranosyloxy)-
4(15) ,10(14)11(13) -guaiat r ien-12,6-ol ide (AR5)及3β-hydroxy-4(15) ,
10(14),11(13)-guaiatrien-12,6-olide (AR6)。本方法以乙腈：水(v/v)為流動相
梯度洗脫，層析管柱為Hypersil ODS-2 (250 mm × 4.6 mm, 5 µm)，柱溫為
30°C，檢測波長為238 nm。本方法之線性、精密性、準確性及特異性均經
評估，回收率95.85 - 98.19%，6種成分均呈良好線性關係（r > 0.9993），
具高度特異性及再現性（RSD%均小於2%），成功地應用於10個檢體的6
種Sesquiterpene Lactones檢測，結果顯示，本方法快速、準確、可靠，且
可應用於光葉苦蕒菜定量分析。

A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of six sesqui-
terpene lactones: 3β-[3-(4-hydroxyphenyl)acetyl-β-D-glucopyranosyloxy]-8β-hydroxy-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR1)
3β-[3-(4-hydroxyphenyl)acetyl-β-D-glucopyranosyloxy]-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR2), 3β-(β-D-glucopyranosyloxy)-
8β-(4-hydroxyphenyl)acetoxy-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR3), 3β-(β-D-glucopyranosyloxy)-8β-(2-hydroxy-3-
methylbutanoyloxy)acetoxy-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR4), 3β-(β-D-glucopyranosyloxy)-4(15),10(14)11(13)-
guaiatrien-12,6-olide (AR5), and 3β-hydroxy-4(15),10(14)11(13)- guaiatrien-12,6-olide (AR6) in the whole plant of Ixeris chinensis. The
separation by gradient elution was performed on Hypersil ODS-2 column (250 mm × 4.6 mm, 5 µm) at 30°C with acetonitrile and water as
the mobile phase, and monitored by absorbance at 238 nm. The parameters of linearity, precision, accuracy, and specificity of the method
were evaluated. The recovery of the method is 95.85-98.19%, and linearity (r > 0.9993) was obtained for all sesquiterpene lactones.
A high degree of specificity as well as repeatability and reproducibility (relative standard deviation values less than 2.0%) were also
achieved. This assay was successfully applied to the determination of six sesquiterpene lactones in ten samples. The results indicated that
the developed assay method was rapid, accurate, reliable and could be readily utilized as a quantitative analysis method for I. chinensis.