環境中微生物常以不同的生長方式與巧妙的分布，使自然生態系統得以維持平衡。然因台灣夏季高溫多濕，促使細菌與黴菌快速繁殖，往往造成諸多環境衛生如飲用水及食品污染，若人類及動植物不經意地遭受致病微生物的感染，則其所造成對人畜的健康及公共衛生的危害常是駭人聽聞的。除普及的衛生教育之外，積極發展新型感測科技以建立快速微生物的鑑定方法，更是避免這些災害發生所刻不容緩的課題。在本文中將聚焦於介紹一種免培養、免標記，且符合多樣性檢測應用之要求，使用結合微流體技術所發展的新型介電泳晶片，透過三維立體結構與巧妙的電極安排和設計，可針對低濃度目標微生物進行快速且連續性分離與捕捉，使其形成高密度聚集體，以增加可檢測濃度，而在晶片捕捉區中特別施予粗糙表面之設計，將更有助於原位拉曼光譜檢測中，提供較高辨識度的拉曼增顯指紋圖譜。

Microorganisms normally exist in the natural environment with different population and nutrient uptake methods under the balanced ecological systems. However, bacteria might pollute our food or water easily owing to it's over growth under the muggy and humid environment. While human beings, animals or even plants are incidentally infected by pathogenic microorganisms, their endangerments to individual and public health are often shocking. In order to prevent and avoid these disasters, it is a great urgency to develop novel culture and label free sensing technologies with rapid detection and identification functions. A novel 3D bio-diagnostic microfluidic chip with continuous bacteria sorting and concentrating functions by dielectrophoresis and in situ bacteria surface enhanced Raman scattering analysis is presented.