台灣地處於亞熱帶地區，為日本腦炎、登革熱、腸病毒的主要疫區。但至今對此類傳染疾病卻無真正有效的藥物可以防治，故了解病毒感染歷程，進而開發有效的抗病毒藥物是現今當務之急。常見與病毒相關之檢測分析，皆須使用實驗室之大型儀器，雖然檢測設備性能優異且結果準確，但伴隨有檢測時間長、所需樣本多、無法即時觀測、成本昂貴等問題。本研究利用微機電製程技術，結合微型懸臂樑感測結構、微流道系統、光學量測、水膠材料，製作一微懸臂樑藥物篩選晶片用以紀錄病毒感染歷程，並對抗體或抗病毒藥物進行抑制作用評估，以達到藥物篩選之目的。本系統針對不同種病毒，在不同病毒濃度的狀況下，成功紀錄其完整感染歷程，發現日本腦炎病毒有明顯的病毒侵入、複製、脫離時期，與其同病毒屬之登革病毒亦有相似之感染歷程。茲此實驗結果，本研究進一步對各種病毒具療效之專一抗體與不具療效之非專一抗體與病毒同時注入系統內，可於數小時內驗證專一抗體確有抗病毒之療效，免除傳統免疫分析之繁瑣與費時。此外，亦對藻類萃取物進行篩選，驗證其確有抵抗登革熱病毒之能力。綜上所述，本系統成功達成紀錄病毒感染歷程與藥物篩選，相信對於藥劑、保健食品、或疫苗開發將有相當助益。

Taiwan, located in the subtropical region, is one of the major epidemic areas of Japanese encephalitis, dengue fever and enterovirus. There is still no effective drug or vaccine that can truly cure the mentioned-above infectious diseases until now. It is the first priority to understand the infection processes of the viruses in order to find effective antiviral drugs or vaccines. Most traditional methods have to use large equipments located in the laboratory to detect or analyse virus. Although they have accurate detection abilities, but also have many disadvantages like long detection time, large volume of samples and reagents, non-real time, expensive cost. This study has proposed a novel system to record completed infection processes of Japanese encephalitis, dengue fever and enterovirus. Meanwhile, the inhibiting ability of antibody or antiviral drug has studied also by the same system. The system is consisted of a biochip manufactured by MEMS technology, optical measurement platform, and biocompatible hydrogel material. The biochip size is 3.15  3.15 cm which is consisted of microcantilever beams with hydrogel and a PDMS microfluidic. The biochip successfully recorded infection processes of different viruses in different concentrations. It was found that Japanese encephalitis had obvious penetration, replication and separation stages. Furthermore, dengue fever virus, which is the same genus as Japanese encephalitis, had a similar infection process. In view of the detection results, for each of the virus mentioned above, the effective specific antibody and the ineffective non-specific antibody were respectively injected into the biochips along with the virus. The antivirus ability of the antibodies was verified within few hours. The tedious work and the time cost by traditional immunoassay were avoided. In addition, the antiviral drug, algae extract, was proved that it could inhibit dengue fever virus for few hours. This study had proved that the biochip and detection system could successfully record completed infection processes for different virus and drug screen. It is believed that the experiment results described above are helpful for drug screen, functional food, and vaccine discovery.