本實驗目的擬觀察絞股藍皀苷對體外培養的CD4淋巴細胞的凋亡及死亡率。抽血培養一名B型肝炎帶原患者的周邊CD4淋巴細胞，運用Annexin V方法將分離的周邊淋巴細胞分空白對照組、絞股藍皀苷組、B型肝炎表面抗原組、B型肝炎表面抗原加絞股藍皀苷組等四組，培養24小時後，再以Annexin V染色及流式細胞儀分析體外培飬淋巴細胞的凋亡及死亡率。結果顯示，絞股藍皀苷組對體外培養的CD4淋巴細胞有降低細胞凋亡及死亡率的作用。而B型肝炎表面抗原組、B型肝炎表面抗原加紋藍皀苷等組有增加CD4淋巴個胞凋亡及死亡率的趨勢，說明B型肝炎表面抗原浩性，會影響CD4淋巴細胞的凋亡。實驗中B型肝炎表面抗原＋紋股藍皀苷組，當絞股藍皀苷的劑量由8.72μg/mL增加爲33μg/mL，經處理24小時後作用於CD4淋巴細胞的凋亡率幾乎接近空白對照組，說明紋股藍皀苷適當的劑量似乎有保護CD4淋巴細胞的作用。

The purpose of study was observed apoptosis and mortality of gypenosides treated peripheral cultured CD4 lymphocyte in vitro. The cultured CD4 lymphocytes were extracted from one Hepatitis B type carrier blood. The separated cultured CD4 lymphocytes by Annexin V method were divided four groups including control group, gypenosides group, HB5Ag group and HBsAg+ gypenosides (8.72 and 33 μg/mL) group. After treated 24 hrs, the Annexin V stain and cell flow cytometry were used to analyzer apoptosis and mortality of cultured CD4 lymphocyte in vitro. The result demonstrated that gypenosides had an effect for decreasing apoptosis and mortality in the cultured CD4 lymphocytes in vitro. The cultured CD4 lymphocytes treated HBsAg and HBsAg adding gypenosides were present an increased level of apoptosis and mortality. This indicated that the activity of HBsAg may be influenced CD4 lymphocytes apoptosis. The experimental group of HBsAg adding gypenosides 33 μg/mL, apoptosis percentage was similar to control group after treated 24 hrs. It revealed that adapted dose of gypenosides had a protection for CD4 lymphocytes.