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中國畜牧學會會誌
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中文摘要
本研究之目的為探討 NCSU-37（North Carolina University 37 Medium）培養液對豬胚 體外生產之影響。在豬胚體外生產系統試驗中，進行 3 次重複試驗，共收集 346 顆 卵丘-卵母細胞複合體進行體外培養。卵母細胞經過 2 階段，合計 46 小時的體外成 熟）in vitro maturation, IVM）培養後，即以冷凍-解凍附睪精液（1 × 105spermatozoa/mL） 進行體外受精（in vitro fertilization, IVF）。IVF 後 3 小時，將受精卵移入第一階段體 外培養（in vitro culture, IVC）液中培養，並於 IVF 後 10 小時觀察原核（pronuclear） 形成及多精入卵的情況。IVF 後 48 小時，移入第二階段 IVC 培養液中繼續培養， 並在 IVF 後的第 6 天進行 Hoechst 33342 染色，以觀察囊胚形成情形及計算囊胚細 胞數。本試驗結果顯示，體外受精率為 51.3±3.1%、多精入卵率為 12.7±3.5%、第 6 天 囊胚率為 11.8±1.7%，囊胚細胞數平均為 47.7±2.5 個。
英文摘要
The study was conducted to investigate the effects of NCSU-37 (North Carolina University 37 Medium) on the in vitro production of porcine embryos. It was performed three replicates of experiments and totally collected 346 cumulus-oocyte complexes in this study. After 46 hours of two-stage in vitro maturation (IVM), these oocytes were subjected to in vitro fertilization (IVF) by the frozen-thawed epididymis semen (1 × 105spermatozoa/mL). After 3 hours of IVF, the fertilized eggs were transferred to the first-stage in vitro culture (IVC) medium. Pronucleogenesis and polyspermy rate were observed after 10 hours of IVF. After 48 hours of IVF, the fertilized eggs were transferred to the second-stage IVC medium. Hoechst 33342 staining was performed to observe the blastocyst formation and calculate the blastocyst numbers after 6 days of IVF. The results showed that the fertilization rate of IVF was 51.3 ± 3.1%, and the polyspermy rate was 12.7 ± 3.5%. Furthermore, the blastocyst formation rate was 11.8 ± 1.7%, and the average numbers of blastocysts was 47.7 ± 2.5 cells.